分享好文：Computer-Assisted Modeling of Antibody Variable Domains
基因工程制药
以下建模方法可以快捷的生成高质量的抗体模型，甚至能够媲美于自动化的服务器提供的模型。按照下面的步骤一步一步操作，即使是生物信息学或建模领域的初学者也能够完成抗体的建模。
材料：
Antibody VH and VL sequences to be modeled.
Computer connected to the Internet.
Swiss-PdbViewer (SPDBV)—Deep Viewer application
方法：
Creating an Organized Structure of Directories for Antibody Modeling.
Sequence Analysis of Antibody VDs.
Homology Search.
VH and VL Primary Model Building Using SPDBV.
VH and VL Association Using Predicted Packing Angle.
CDR-H3 Refinement.
Verification of Potentially Problematic Conformations.
Model Visualization.
Quality Perspective of the Resulting Models: RMSD-to-Native Test.
